Jing Liu

In this study, we observed that BMSCs transplantation could alleviate inflammatory activitiesand functional changes in lungafter MCAo induced ischemic stroke. We also detected inflammation associated gene expression at multiple time points and found a significantdecrease of TNFαin the lung after BMSCs transplantation

Forty-five adult male Sprague–Dawley (SD) rats (weighing 180–220g) were provided by the Center of Experimental Animals, Sichuan University. All animal care, breeding, and testing procedures were confirmed to the principle of <Guidance suggestions for the care and use of laboratory animals> promulgated by Ministry of Science and Technology of the People’s Republic of China in 2006, and were approved by the Animal Care and Use Committee, Sichuan, University, Chengdu, China. All animals were housed in individual cages in a temperature (21–25 °C) and humidity (45–50%)-controlled room with a 12-h light/dark cycle and ad libitum access to pellet chow and water. Animals were randomly divided into three groups: sham operation group, cerebral ischemia group and BMSCs transplantation group, which are showed in Fig1 with their uses respectively.

Permanent focal cerebral infarction was introduced by bipolar coagulation of the left MCA as described previously[10]. Under 3.6% chloral hydrate (1ml/100g) intraperitoneally injection, left common, internal and external carotid arteries were exposed through a midline neck incision and were carefully dissected from the surrounding tissues under an operating microscope. After electrocoagulation of the external and common carotid arteries, a 3–0 silicon rubber-coated monofilament (Shadong Biotech, Beijing, China) was inserted through the common carotid artery into the internal carotid artery 18 to 20 mm beyond the carotid bifurcation to the base of the middle cerebral artery, while 10mm in sham-operated group. The pterygopalatine branch of the internal carotid artery was exposed before the insertion in order to avoid the filament turning into it. Rectal temperature was maintained at 36.5–37°C using a heat lamp during the operation and for 2 hours after MCAo, and breath and heart rate were monitored at any time.

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